Life moves. Many neuronal and physiological processes occur at very high speeds, making it difficult to accurately capture their

to the plane of illumination and can easily add up to harmful amounts across the volume. Lightfield 4D works differently:

Living samples can be imaged over long periods of time at high temporal density. This combination of outstanding 3D imaging

Typically, acquisition time of large volumes is the critical factor that limits the throughput of imaging. Acquiring a large volume
with a single image snap speeds up your experiments by multitudes. The unmatched speed with which Lightfield 4D captures
multi-color volumes can be used to increase the productivity of experiments in a variety of ways: Image and analyze more
samples than ever before in every session, immediately improving experimental statistics. Compare multiple different sample
cohorts of wild type and genetically modified phenotypes, or samples with different drug treatments. Instead of hours, only
minutes are spent collecting the data you need, leaving you more time for advanced analysis and investigation of your datasets.

Laser scanning microscopes (LSM) have proven to be the most versatile microscopy systems. They combine super-resolution
and spectral imaging with high-quality optical sectioning of large samples, along with the capability to incorporate additional
fluorescent information and molecular dynamics measurements. Take your experiments to the next level by pairing this remark
able flexibility with the gentle and instant volume imaging of Lightfield 4D: Monitor neuronal activity in 3D at high speed, and
supplement this with super-resolution structural details captured with Airyscan. Track macrophage movement during a wound
healing assay and add high-resolution details of the wound site to your investigation. Leverage the photomanipulation capabili-
ties of your LSM for bleaching, photoactivation, photoconversion, or ablation experiments, followed by gentle volume imaging.
Accomplish all of this on the same microscope as part of the same experiment without ever moving your sample.

ously. Often, calcium signaling is recorded in a single plane only or in a very small
volume. Yet, to understand the functioning of neuronal circuitry, it is essential to

speed to track neuronal activation. Simultaneous firing of neurons sitting 100 μm or
more apart can be captured, giving completely new insights into neuronal circuitry.
To have a closer look at the specific neuronal morphology, high-resolution images

must be done in 4D, as both volume and time are essential
for investigating living systems. This concept is not new; many
optical sectioning techniques have been developed over the

information at the same instant. Each of these different views
provides both spatial and angular information which serves

Not for therapeutic use, treatment or medical diagnostic evidence. Not all products are available in every country. Contact your local ZEISS representative for more information.
EN_41_012_338 | Version 1.0 | CZ 03-2025 | Design, scope of delivery, and technical progress subject to change without notice. | © Carl Zeiss Microscopy GmbH